Prepare Protein At The Beginnning Of The Day A Question Involving Beer's Law?

A question involving beer's law? - prepare protein at the beginnning of the day

An unknown protein was isolated in his laboratory. Was found
This protein contains 172 amino acids and contains no
Tryptophan or phenylalanine. They were asked to determine the possibility of
Protein-tyrosine. Prepare a 50μM solution of pure
Protein body in a cell with a sample of 1 cm path length measured,
Absorbance at 280 nm in the UV-VIS spectrophotometer.
The absorption of the solution is 0372nd How tyrosine
present in this protein? (Molar absorption coefficient = 1225 M-1 cm-1).